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KMID : 0603520030080030195
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Journal of Korean Association of Cancer Prevention
2003 Volume.8 No. 3 p.195 ~ p.200
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Identification of Human Peptidylglycine Alpha-amidating Monooxygenase(PAM) in Posriasis by DDRT-PCR
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Kwon Yoo-Wook
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Abstract
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Psoriasis is a chronic relapsing and angiogenic skin disease characterized by variable clinical features. However, not only pathogenetic process but also etiological factors remain to be proven, In order to identify genes differentially expressed in psoriasis, we performed mRNA differential display analysis using total RNA extracted from normal and psoriatic primary cultured cells. Of the differentially expressed genes from psoriasis, some of cDNA fragments were cloned and sequenced. The expression patterns of these clones from psoriatic primary cells were confirmed by reverse northern blot analysis. Up-regulated gene from lesional keratinocytes of psoriasis was identified as human peptidylglycine alpha-amidating monooxygenase (PAM). Up-regulated expression of PAM in psoriasis may contribute to pathogenesis of psoriasis.
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KEYWORD
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Psoriasis, PAM, Differential display analysis
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